Kmt2d knockdown resulted in Akt/β-catenin pathway activation in vitro. a and b) The relative p-Akt (Ser473) protein level decreased in the knockdown group. c and d) The relative p-β-catenin (Ser552) protein level decreased in the knockdown group. e and f) The relative cyclin D1 protein level decreased in the knockdown group. The band density was quantified and expressed as the relative gray value (compared with the control) by arbitrarily setting the control value as 1. Data were pooled from 3 independent experiments, and the results were represented as mean ± SD. *P < 0.05, **P < 0.001, ***P < 0.0001.
|