FIGURE

Fig. 2

ID

ZDB-FIG-240307-12

Publication

Russell et al., 2021 - Pathogenic effect of TP73 Gene Variants in People With Amyotrophic Lateral Sclerosis

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Fig. 2

ALS Variants in TP73 Render p73 Dysfunctional in C2C12 Differentiation Demonstrated by Quantification of MHC Staining Intensity and Myotube Width Compared to EGFP (A) Quantification of normalized myosin heavy chain (MHC) staining in differentiated C2C12s, with or without exposure to doxycycline, expressing enhanced green fluorescent protein (EGFP), wild-type (WT) ?N-p73?, or mutant ?N-p73. WT ?N-p73? expression results in inhibited C2C12 differentiation shown by reduced MHC immunohistochemistry staining compared to EGFP. ?N-p73?Mut1+3 expression fails to inhibit C1C12 differentiation shown by no difference in MHC staining compared to EGFP (**p < 0.001, Wilcoxon rank-sum test with Bonferroni correction). (B) Normalized myotube width quantification. ?N-p73?Mut2-4 expression results in myotubes with significantly larger width compared to EGFP. ALS = amyotrophic lateral sclerosis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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