Figure 5

AP alleviates inflammatory response induced by LPS treatment in zebrafish. (a) Representative images of posterior areas of Sudan Black B-stained zebrafish embryos after LPS induction and AP treatment at 3 dpf. The upper panel shows the control group with DMSO (0.025%) treatment, the middle panel shows LPS inflammation group, and the bottom panel shows the LPS inflammation group after treatment with 10 µg/mL AP. (b) Quantification of neutrophils in the posterior region of zebrafish after LPS induction and AP treatment. (c) Representative images of the caudal hematopoietic tissues after LPS induction and AP treatment at 3 dpf. Whole-mount in situ hybridization was conducted using an mpx probe. (d) Representative images of anterior Sudan Black B-stained zebrafish embryos following direct LPS injection and AP treatment at 3 dpf. (e) Quantification of the number of neutrophils in the anterior zebrafish embryos after LPS induction and AP treatment. All graphs represent the mean ± S.E.M. of individual values. P-values were calculated using an unpaired two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.