FIGURE

Figure 4

ID

ZDB-FIG-240305-24

Publication

Lee et al., 2024 - Aiouea padiformis extract exhibits anti-inflammatory effects by inhibiting the ATPase activity of NLRP3

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Figure 4

AP blocks NLRP3 inflammasome assembly by hindering ATPase activity of NLRP3. (a) HEK 293FT cells were transfected with NLRP3-Myc and treated with or without AP for 2 h. The interaction between NLRP3 and NEK7 was analyzed by immunoprecipitation and immunoblot. (b) The ATPase activity of NLRP3 with or without AP was measured by luminescence using the ADP-Glo™ Max assay. (c) LPS-primed THP-1 cells were treated with AP for 2 h and activated with nigericin (10 μM) for 30 min. The cell pellet was cross-linked by DSS (2.5 mM) for 30 min. ASC oligomerization was analyzed by immunoblot. (d,e) LPS-primed J774A.1 cells treated with AP for 2 h and activated with nigericin (10 μM) for 30 min. Representative immunofluorescence images of ASC speck formation (indicated by arrows) were taken by a confocal laser-scanning microscope (Carl Zeiss, LSM710, scale bar, 20 μm) (d). Graph representing the number of cells containing ASC specks in all treatments (e).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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