Fig. 5

Tracking of the basal cell behaviour in adult fish. (A) Tracking of the itgb4:cre-labelled cells at a single cell resolution in adult fish fin (3 months old). The images were taken from the same fin region of the fish. White arrows indicate the posterior side (P). Experimental procedure is shown at the top. Arrowheads indicate representative corresponding cells between these panels. Most of the labelled clones were quiescent in a time window between 2-14 dpl. Scale bar: 200 µm. (B) Ratios of clones with respective proliferation patterns in the adult fin epidermis (n=897 clones from six adult zebrafish). (C) Confocal optical sections of itgb4:cre-labelled cells in the adult fin epidermis. Basal cells were labelled with tamoxifen (TAM) treatment. The basal cells did not produce the non-basal cells. Scale bar: 50 µm. Dashed lines indicate basement membrane.