Fig. S8
- ID
- ZDB-FIG-240206-15
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- Zhao et al., 2023 - Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth
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Cryo-EM densities of the glycosyl-Qs and codon-anticodon interactions of human tRNAs at the P-site, related to Figure 6 (A) Unpostprocessed maps (gray mesh) of manQ (level 0.0104, EMDB: EMD-36179) and galQ (level 0.012, EMDB: EMD-36181) are superimposed on each model structure (manQ: 8JDK, galQ: 8JDM) in human 80S ribosome. (B) Unpostprocessed maps (gray mesh) of manQ (level 0.0075, EMDB: EMD-33662) and galQ (level 0.0072, EMDB: EMD-33664) are superimposed on each model structure (manQ: 7Y7E, galQ: 7Y7G) in E. coli 70S ribosome. (C–J) Close-up views (C, D, G, and H) and upper side views (E, F, I, and J) of the codon-anticodon interaction at the P-site of the ribosome with human tRNAAsp bearing Q34 (yellow) and GAU codon (magenta) (PDB: 8JDJ ) (C and E), human tRNAAsp bearing manQ34 (yellow) and GAU codon (magenta) (PDB: 8JDK) (D and F), human tRNATyr bearing galQ34 (yellow) and UAU codon (magenta) in the classical state (PDB: 8JDL) (G and I), and in the hybrid state (PDB: 8JDM) (H and J). 18S rRNA is colored cyan. Hydrogen bonds are shown as dotted lines. |
Reprinted from Cell, 186(25), Zhao, X., Ma, D., Ishiguro, K., Saito, H., Akichika, S., Matsuzawa, I., Mito, M., Irie, T., Ishibashi, K., Wakabayashi, K., Sakaguchi, Y., Yokoyama, T., Mishima, Y., Shirouzu, M., Iwasaki, S., Suzuki, T., Suzuki, T., Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth, 5517-5535.e24, Copyright (2023) with permission from Elsevier. Full text @ Cell