Fig. S7

Cryo-EM analyses of codon-anticodon interactions of human tRNAs at the A-site of E. coli 70S ribosome complexes, related to Figure 6 (A) Close-up view of the codon-anticodon interaction at the A-site of the ribosome with human tRNAAsp bearing Q34 (green) and GAU codon (magenta) (PDB: 7Y7D ). 16S rRNA is colored cyan. Hydrogen bonds are shown as dotted lines. (B, C, and E) Cryo-EM structure of the codon-anticodon interaction at the A-site between human tRNAAsp bearing Q34 (green) and the GAU codon (PDB: 7Y7D ) (B), human tRNAAsp bearing manQ34 (green) and the GAU codon (PDB: 7Y7E) (C), and human tRNATyr bearing galQ34 (green) and the UAU codon (PDB: 7Y7G ) (E), viewed from the major groove side (upper) and from the upper side (lower). Hydrogen bonds are shown as dotted lines. (D and F) Cryo-EM structure of the codon-anticodon interaction at the A-site between human tRNAAsp bearing manQ34 (green) and the GAC codon (PDB: 7Y7F ) (D), and human tRNATyr bearing galQ34 (green) and the UAC codon (PDB: 7Y7H) (F), viewed from the upper side. Hydrogen bonds are shown as dotted lines.