Fig. S8

Fig. S8 Cryo-EM densities of the glycosyl-Qs and codon-anticodon interactions of human tRNAs at the P-site, related to Figure 6 (A) Unpostprocessed maps (gray mesh) of manQ (level 0.0104, EMDB: EMD-36179) and galQ (level 0.012, EMDB: EMD-36181) are superimposed on each model structure (manQ: 8JDK, galQ: 8JDM) in human 80S ribosome. (B) Unpostprocessed maps (gray mesh) of manQ (level 0.0075, EMDB: EMD-33662) and galQ (level 0.0072, EMDB: EMD-33664) are superimposed on each model structure (manQ: 7Y7E, galQ: 7Y7G) in E. coli 70S ribosome. (C–J) Close-up views (C, D, G, and H) and upper side views (E, F, I, and J) of the codon-anticodon interaction at the P-site of the ribosome with human tRNAAsp bearing Q34 (yellow) and GAU codon (magenta) (PDB: 8JDJ ) (C and E), human tRNAAsp bearing manQ34 (yellow) and GAU codon (magenta) (PDB: 8JDK) (D and F), human tRNATyr bearing galQ34 (yellow) and UAU codon (magenta) in the classical state (PDB: 8JDL) (G and I), and in the hybrid state (PDB: 8JDM) (H and J). 18S rRNA is colored cyan. Hydrogen bonds are shown as dotted lines.