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Stable shank3ab mutant lines exhibit hyporeactivity and hypoactivity following a light to dark transition. a) shank3ab N-terminal and C-terminal mutants were designed to target regions with known deleterious mutations in individuals with PMS. b) Trace line graphs showing four cycles of 5 minutes lights-on to lights-off. Checkered boxes on the x-axis represent lights on and off. c) Lights on to off paired comparison, highlighting no significant change in activity of shank3ab N terminal mutants during the first 30 sec lights-off. d) Box plots showing first 30 sec lights-off activity. e) Box plots showing activity across the full 5 minutes lights-off. Box plots represent 25th and 75th percentile, and median, with min to max whiskers. Sample sizes: WT = 50, shank3 N = 65, shank3 C = 44. p values; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.
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