Fig. 5

DHX38 inhibition slowed down ongoing DNA synthesis and caused cell-cycle arrest (A) FACS analysis of PI (propidium iodide) staining in HEK293 cells treated with either control siNC or siDHX38. N = 4 separate experiments. Data was shown as mean ± SD. n.s., no significance; ∗∗p < 0.01 as indicated. (B and C) The experimental scheme of DNA fiber assay (left) and representative DNA fibers from the identified conditions (middle), quantified by CldU track length (right). For each condition the lengths of the CIdU tracks of n ≥ 100 fibers were quantified. Scale bar, 3 μm. Data was shown as mean ± SD. ∗∗∗∗p < 0.0001 as indicated. (D and E) Left: Western blotting of total HEK293 cell extracts upon asynchronously growing (D) or serum starvation (E) after DHX38 knockdown. Cell cycle distribution was verified using FACS analysis (right panel). (F and G) Immunofluorescence images of γH2AX fluorescence in asynchronously growing or serum starvation HEK293 cells after DHX38 knockdown. Quantification of at least 100 cells from three independent experiments. Scale bar, 30 μm. Data was shown as mean ± SD. ∗∗∗∗p < 0.0001 as indicated.