Figure 7

Altered angiogenic hyaloid vasculature caused by aldh9a1b knockout and tt‐DDE treatment can be rescued by metformin, rosiglitazone and carnosine (A) Representative confocal images of hyaloid vasculature showed vascular alterations and beneficial effects in aldh9a1b^−/− zebrafish larvae treated with MF, RG, and CAR. White scale bar = 50 µm. B) Quantification of hyaloid branch points and sprouts formation showed increased angiogenic vasculature in aldh9a1b^−/‐ can be rescued by metformin, rosiglitazone and carnosine. One data point means one hyaloid per larva. C) Representative confocal images of hyaloid vasculature showed vascular alterations and beneficial effects in aldh9a1b^+/+ larvae co‐incubated with tt‐DDE and MF, RG, and CAR. White scale bar = 50 µm. D) Quantification of hyaloid branch points and sprout formation showed that increased angiogenic vasculature induced by tt‐DDE can be rescued by metformin, rosiglitazone, and carnosine. One data point means one hyaloid per larva. The bars indicate mean ± SD values. Statistical analysis was performed by one‐way ANOVA. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. MF, metformin; RG, rosiglitazone; CAR, carnosine; PK, PK11195.