Figure 6

Impaired glucose homeostasis in aldh9a1b^−/‐ and tt‐DDE treated zebrafish (A) The whole‐body glucose was significantly increased in tt‐DDE treated aldh9a1b^+/+ larvae at 5 dpf. In aldh9a1b^−/‐ larvae, whole‐body glucose was also increased, but not significantly, n = 4/6. B) Blood glucose of aldh9a1b^−/− adults did not show any alteration in fasting state but was significantly increased in the 2‐hour postprandial state. C) Graphical overview of key biological processes in glucose metabolism. D) mRNA levels of key enzymes in glucose metabolism showed alterations of glycolysis, gluconeogenesis, and glycogenesis in aldh9a1b^−/− and in tt‐DDE treated aldh9a1b^+/+ larvae at 5dpf, n = 6/7. E,F) mRNA levels of key enzymes in glucose metabolism showed alterations of glycolysis, gluconeogenesis and glycogenesis in the livers (E) and muscles (F) of aldh9a1b^−/− zebrafish, n = 6/5. mRNA Expression was quantified by RT‐qPCR and normalized to b2m. Each data point in this figure represented 20 larvae or one fish. The bars indicate mean ± SD values. Statistical analysis was performed by Student's t‐test and one‐way ANOVA, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.