Fig. 7

ZAK? regulates hematopoiesis in zebrafish through the Nlrp1 inflammasome A?K Number of neutrophils (B, D, H), erythrocytes (F) and macrophages (J), and caspase?1 activity (K) in 2 dpf larvae either treated from 1 to 2 dpf by bath immersion with 1 ?M nilotinib and/or 100 ?M anisomycin (A?D) or obtained by injecting one?cell stage embryos with standard or zaka crRNAs/Cas9 complexes (E?K). Representative images of neutrophils (A, C, G), erythrocytes (E) and macrophages (I) are also shown. Each dot represents one individual and the mean ± SEM for each group is also shown. P values were calculated using one?way ANOVA and Tukey's multiple range test (B) or Student's t?test (D, F, H, J, K). Data are shown as the means ± SEM of two technical replicates in (K). n.s., non?significant; *P < 0.05; **P < 0.01; ***P < 0.001.