Figure 4.

Pharmaco-behavioural analyses of behaviour-modifying compounds.

(A) Clustered heatmap of the behavioural fingerprints for the 176 hits of the screen, showing at least one behaviour measure with |SSMD|≥2. Clustering distance = ward, standardized Euclidean, colour/cluster threshold = 9.5. This led to the re-identification of haloperidol and clozapine as habituation modifiers (light blue arrows). (B) Clustered correlogram of the Pearson correlation coefficients for the different measured components of behaviour across hits (same data as A) revealing the independence or co-modulation of behaviours. Clustering distance = average, correlation, colour/cluster threshold = 1.5. (C) Subsets of clustered heatmap from (A), highlighting the similar phenotypes exhibited by (i) GABA receptor antagonists and (ii, iii) melatonin receptor agonists, oestrogen receptor agonists, progesterone receptor agonists, and peroxisome proliferator-activated receptor alpha (PPARα) agonists. Heatmap is cropped to the first three columns of (A), depicting the strictly standardized mean difference (SSMD) of response probability relative to vehicle controls.