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Dynamic pdgfrb expression in the injured area during regeneration. (A,A′) pdgfrb expression early during heart regeneration. (A) Tg(pdgfrb:Citrine; fli1a:DsRed) fish hearts at 1, 3, 7 and 14 dpa. White dashed line indicates the injured area. White dotted line indicates the pdgfrb-expressing area. In the insets, white arrowheads indicate pdgfrb expression in mural cells and the yellow arrowheads non-mural cell pdgfrb expression. (A′) Quantification of the pdgfrb+ area as a percentage of the imaged heart area. Error bars represent s.d. *P≤0.05 (one-way ANOVA). 1 day, 3 days, 30 days: n=3; 7 days: n=5; 10 days, 14 days: n=4. (B) pdgfrb expression in epicardium. Tg(pdgfrb:Citrine; fli1a:EGFP; tcf21:DsRed) fish hearts imaged as whole-mount preparations and as sections in uncut, 1, 3 and 7 dpa fish. White arrowheads indicate pdgfrb expression in mural cells; yellow arrowheads non-mural cell pdgfrb expression.
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