Fig. 7

The IL1β-NFκB axis is activated in the migrated ileal enterocytes to promote transdifferentiation (A and B) At 0 hpt, in contrast to the uninjured control, the expression of il1b (A) and the activity of NF-κB (B) became activated in the migrated ileal enterocytes, as exhibited by FISH and NFKB:GFP reporter transgene, respectively. (C–H) In contrast to Tg(fabp2:DenNTR; fabp6:dsRed) transgenic larvae injured by MTZ, the il1b mutation or treatment with JSH-23 was ineffective in the anterior migration of the DsRed+ ileal enterocytes at 0 hpt (C and F) but exhibited defective transdifferentiation into the Dendra2+ jejunal enterocytes at 48 hpt (D and G). Graphs show quantification of ratio of Dendra2+ among DsRed+ enterocytes in jejunum for il1b mutant (E) and JSH-23 treatment (H). Data are presented as mean ± SEM. ∗∗∗∗p < 0.0001, two-tailed unpaired t test. (I) Treatment with ceruletide, an agonist of NF-κB, promoted the transdifferentiation from migrated ileal enterocytes to the jejunal enterocytes at 16 hpt (MTZ, n = 28; MTZ + ceruletide, n = 26). Scale bars, 50 μm. See also Figures S6 and S7.