PUBLICATION

Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish

Authors
Wei, X., Tan, X., Chen, Q., Jiang, Y., Wu, G., Ma, X., Fu, J., Li, Y., Gang, K., Yang, Q., Ni, R., He, J., Luo, L.
ID
ZDB-PUB-230622-39
Date
2023
Source
Cell Reports   42: 112660112660 (Journal)
Registered Authors
He, Jianbo, Luo, Lingfei
Keywords
CP: Stem cell research, NF-?B, cell migration, enterocyte regeneration, intestinal failure, jejunum, stem cell, transdifferentiation
MeSH Terms
  • Jejunum*
  • Cell Transdifferentiation
  • Necrosis
  • Enterocytes*
  • Intestinal Mucosa
  • Zebrafish
  • Animals
(all 7)
PubMed
37342912 Full text @ Cell Rep.
Abstract
A major cause of intestinal failure (IF) is intestinal epithelium necrosis and massive loss of enterocytes, especially in the jejunum, the major intestinal segment in charge of nutrient absorption. However, mechanisms underlying jejunal epithelial regeneration after extensive loss of enterocytes remain elusive. Here, we apply a genetic ablation system to induce extensive damage to jejunal enterocytes in zebrafish, mimicking the jejunal epithelium necrosis that causes IF. In response to injury, proliferation and filopodia/lamellipodia drive anterior migration of the ileal enterocytes into the injured jejunum. The migrated fabp6+ ileal enterocytes transdifferentiate into fabp2+ jejunal enterocytes to fulfill the regeneration, consisting of dedifferentiation to precursor status followed by redifferentiation. The dedifferentiation is activated by the IL1β-NFκB axis, whose agonist promotes regeneration. Extensive jejunal epithelial damage is repaired by the migration and transdifferentiation of ileal enterocytes, revealing an intersegmental migration mechanism of intestinal regeneration and providing potential therapeutic targets for IF caused by jejunal epithelium necrosis.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
il1bCRISPR11-il1bCRISPR
il1bCRISPR12-il1bCRISPR
1 - 2 of 2
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Fish
No data available
Antibodies
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
CFPEFGCFP
CreEFGCre
Dendra2EFGDendra2
DsRedEFGDsRed
DsRedxEFGDsRedx
EGFPEFGEGFP
GFPEFGGFP
KaedeEFGKaede
mCherryEFGmCherry
NTREFGNTR
1 - 10 of 12
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Mapping
No data available
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Citation
Wei, X., Tan, X., Chen, Q., Jiang, Y., Wu, G., Ma, X., Fu, J., Li, Y., Gang, K., Yang, Q., Ni, R., He, J., Luo, L. (2023) Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish. Cell Reports. 42:112660112660.