PUBLICATION

Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish

Authors
Wei, X., Tan, X., Chen, Q., Jiang, Y., Wu, G., Ma, X., Fu, J., Li, Y., Gang, K., Yang, Q., Ni, R., He, J., Luo, L.
ID
ZDB-PUB-230622-39
Date
2023
Source
Cell Reports   42: 112660112660 (Journal)
Registered Authors
He, Jianbo, Luo, Lingfei
Keywords
CP: Stem cell research, NF-?B, cell migration, enterocyte regeneration, intestinal failure, jejunum, stem cell, transdifferentiation
MeSH Terms
  • Intestinal Mucosa
  • Animals
  • Enterocytes*
  • Necrosis
  • Jejunum*
  • Zebrafish
  • Cell Transdifferentiation
(all 7)
PubMed
37342912 Full text @ Cell Rep.
Abstract
A major cause of intestinal failure (IF) is intestinal epithelium necrosis and massive loss of enterocytes, especially in the jejunum, the major intestinal segment in charge of nutrient absorption. However, mechanisms underlying jejunal epithelial regeneration after extensive loss of enterocytes remain elusive. Here, we apply a genetic ablation system to induce extensive damage to jejunal enterocytes in zebrafish, mimicking the jejunal epithelium necrosis that causes IF. In response to injury, proliferation and filopodia/lamellipodia drive anterior migration of the ileal enterocytes into the injured jejunum. The migrated fabp6+ ileal enterocytes transdifferentiate into fabp2+ jejunal enterocytes to fulfill the regeneration, consisting of dedifferentiation to precursor status followed by redifferentiation. The dedifferentiation is activated by the IL1β-NFκB axis, whose agonist promotes regeneration. Extensive jejunal epithelial damage is repaired by the migration and transdifferentiation of ileal enterocytes, revealing an intersegmental migration mechanism of intestinal regeneration and providing potential therapeutic targets for IF caused by jejunal epithelium necrosis.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ca2TgTransgenic Insertion
    cq39TgTransgenic Insertion
      cq161TgTransgenic Insertion
        cq162TgTransgenic Insertion
          cq163TgTransgenic Insertion
            cq164TgTransgenic Insertion
              cq165TgTransgenic Insertion
                cq166TgTransgenic Insertion
                  cq167TgTransgenic Insertion
                    cq168TgTransgenic Insertion
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      il1bCRISPR11-il1bCRISPR
                      il1bCRISPR12-il1bCRISPR
                      1 - 2 of 2
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                      Fish
                      No data available
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab2-dendra2polyclonal
                        		IgGRabbit
                        Ab5-cdh1polyclonal
                          		Rabbit
                          Ab-5F11monoclonal
                            		IgG1Mouse
                            Ab11-casp3monoclonal
                              		IgGRabbit
                              1 - 4 of 4
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                              Orthology
                              No data available
                              Engineered Foreign Genes
                              Marker Marker Type Name
                              CFPEFGCFP
                              CreEFGCre
                              Dendra2EFGDendra2
                              DsRedEFGDsRed
                              DsRedxEFGDsRedx
                              EGFPEFGEGFP
                              GFPEFGGFP
                              KaedeEFGKaede
                              mCherryEFGmCherry
                              NTREFGNTR
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                              Mapping
                              No data available
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                              Citation
                              Wei, X., Tan, X., Chen, Q., Jiang, Y., Wu, G., Ma, X., Fu, J., Li, Y., Gang, K., Yang, Q., Ni, R., He, J., Luo, L. (2023) Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish. Cell Reports. 42:112660112660.