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cdon and boc sensitize zebrafish embryos to a reduction in hedgehog signal by cyclopamine treatment. (A) Lateral views of representative images show slow-twitch muscle fiber morphology in 24 hpf embryos treated with either 20µM cyclopamine or an equivalent volume of ethanol as a control. cdon and boc mutants exhibit more severe phenotypes when treated with cyclopamine, as evidenced by gaps in segments (arrows), and complete loss of slow-twitch muscles in the double mutants. (B) Quantification of average number of slow-twitch muscle fibers per segment shows that cdon and boc mutations are sensitizing embryos to cyclopamine treatment. All images and data are taken from segments at the level of the yolk extension. Horizontal line in box plot indicates the median. Lower and upper edges of boxes indicate the first and third quartiles, respectively. Whiskers indicate minimum and maximum values. Significance values are from Tukey pairwise post-hoc tests (*P<0.05, ***P<0.001).
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