PUBLICATION
Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish
- Authors
- Lencer, E., Rains, A., Binne, E., Prekeris, R., Artinger, K.
- ID
- ZDB-PUB-230701-32
- Date
- 2023
- Source
- Development (Cambridge, England) 150(14): (Journal)
- Registered Authors
- Artinger, Kristin Bruk, Lencer, Ezra
- Keywords
- Adaxial Cell, Cell Guidance, Cell Migration, Extracellular Matrix, Hedghog Signaling, Neural Crest Cell
- MeSH Terms
-
- Animals
- Cell Adhesion Molecules/genetics
- Cell Differentiation
- Cell Movement/genetics
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism
- Muscle Development/genetics
- Mutation/genetics
- Neural Crest*
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 37390228 Full text @ Development
Citation
Lencer, E., Rains, A., Binne, E., Prekeris, R., Artinger, K. (2023) Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish. Development (Cambridge, England). 150(14):.
Abstract
The transmembrane proteins cdon and boc are implicated in regulating hedgehog signaling during vertebrate development. Recent work showing roles for these genes in axon guidance and neural crest cell migration suggest that cdon/boc may play additional functions in regulating directed cell movements. We use novel and existing mutants to investigate a role for cdon and boc in zebrafish neural crest cell migration. We find that single mutant embryos exhibit normal neural crest phenotypes, but that neural crest migration is strikingly disrupted in double cdon;boc mutant embryos. We further show that this migration phenotype is associated with defects to the differentiation of slow-twitch muscle cells, and the loss of a Col1a1a containing extracellular matrix, suggesting that neural crest defects may be a secondary consequence to defects in mesoderm development. Combined, our data add to a growing literature showing that cdon and boc act synergistically to promote hedgehog signaling during vertebrate development, and suggest that the zebrafish can be used to study the function of hedgehog receptor paralogs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping