Fig. 3

Transcriptomic analysis of rods and newly formed rods in the WT-P23H integrated dataset reveal an increased response to oxidative stress and misfolded proteins. A Overlap of UMAP dimensionality reduction and visualization of rod transcriptomes from WT and P23H transgenic fish. B Violin plots showing levels of expression of canonical marker genes in WT and P23H rods. C–E Violin plots showing expression levels of differentially expressed genes (DEGs) involved in various functional pathways including oxidative stress, response to misfolded proteins, ciliary transport, synaptic remodeling, and metabolism in WT and P23H rods. F Dot plot showing the expression level and the percentage of cells expressing DEGs involved in different functional pathways in WT and P23H. G Violin plots showing expression levels of DEGs involved in proteasome and ubiquitin-mediated degradation pathway and stress response markers in WT and P23H newly formed rods