Fig. 7

Restoration of RSD-induced L/M deficit by overexpression of OGT. An vOGT virus was injected into the brains of zebrafish. A Schematic illustrating the experimental design of vOGT (vOGT) injection and induction of either SD or RSD. B–D Representative confocal images (40×) of DAPI (blue), OGT (B, green), O-GlcNAc (C, green), OGA (D, green), and merged immunofluorescence staining in the Dl region of zebrafish telencephalon are displayed. Enlarged images are presented in the white boxes. The graphs represent the quantitative results for each antibody with normalization by the DAPI levels (n = 3/group). For statistical analysis, the Kruskal–Wallis test with the original FDR of Benjamini and Hochberg was performed (*p < 0.05, **p < 0.01 versus Con, ^##p < 0.01 versus RSD). E Graphs represent fear context L/M test results for the SD or RSD group with or without vOGT injection. They illustrate the altered crossing times compared to those observed during the first learning session and display the mean ± SEM (n = 8 ~ 10/group). For statistical analysis, within-group comparisons, the Friedman ANOVA test was performed with the original FDR of Benjamini and Hochberg (^ap < 0.05, ^aap < 0.01 versus 1st learning). Between-group comparisons, a two-way ANOVA with Tukey’s multiple comparisons test was performed (*p < 0.05, **p < 0.01 versus Con, ^##p < 0.01 versus SD or RSD)