Fig. 5

Surface proteins modulate neutrophil dissemination of Fn nucleatum ex vivo and in vivo. a) Graph compares the velocity of neutrophils in response to fMLP to cell-laden with wild-type (WT) Fnn or Fnn with deletion of the Fap2 or RadD genes. Cells laden with mutant bacteria maintained higher velocities than those carrying WT bacteria. Each spot represents the average velocity of a neutrophil track. Data are pooled from N = 3 experiments. Red horizontal line represents the median and the dashed lines represent the first and third quartile b) Graph shows that the distribution of zebrafish neutrophils laden with bacteria in vivo at 5 hpi was significantly higher in larvae infected with Fnn Fap2 mutant bacteria. Each spot represents the average distribution of laden neutrophils within an infected zebrafish. Data pooled from N = 3 experiments. Red horizontal line represents the median and the dashed lines represent the first and third quartile (One-way ANOVA with Tukey?s multiple comparisons test.).