Fig. 4

Leukocytes are required for disseminating Fnn in vivo. a) Representative images of Control-MO and spi1-MO/csf3r-MO treated larvae 5 hours post-infection (hpi) with F. nucleatum. the Control-MO animal has GFP-expressing neutrophils (green fluorescence), which engulf and distribute AleaFluor-594 labeled bacteria (red fluorescence). Animals injected with a spi1-MO/csf3r-MO cocktail have suppressed leukocyte development. Injected bacteria remain confined at the infection bolus in the developing ear (otic vesicle). b) Graph shows the average distance of fluorescent bacteria from the infection bolus for different bacterial species in Control-MO and spi1-MO/csf3r-MO-injected larvae at 5 hpi. Dispersion of Fnn was significantly reduced at 5 hpi in the absence of leukocytes. Statistics: One-way ANOVA with Tukey?s multiple comparisons test. Each point is the average distance of bacteria from the infection bolus within a fish after 5 hours. Red horizontal line represents the median and the dashed lines represent the first and third quartile.