Figure 2.

High-definition recording and measurement of animal locomotion using SAMPL

(A) Example of a recorded frame with a Drosophila melanogaster (white box) in the SAMPL apparatus. Dashed line indicates heading of the fly relative to vertical up (north). Imaging was performed at 166 Hz with 1,200 × 1,216 pixels. Same as follows.

(B) Example of an epoch of a walking fly. Walking speed and heading are plotted as a function of time. Gray and cyan lines mark resting and grooming periods, respectively (Video S2).

(C) Example of a recorded frame with a Caenorhabditis elegans (white box) in the SAMPL apparatus. Dashed line indicates approximated angle of the worm relative to vertical.

(D) Example of an epoch of a swimming worm. z position and approximated angle are plotted as a function of time. Cyan vertical lines label the time when the plane of movement is perpendicular to the imaging plane (Video S2).

(E) Example of a recorded frame with a 7 dpf Danio rerio larva (white box) in the SAMPL apparatus. Pitch angle is determined as the angle of the trunk of the fish (dashed line) relative to horizontal. Positive pitch indicates nose-up posture, whereas negative pitch represents nose-down posture.

(F) Example of an epoch containing multiple swim bouts (arrows). Swim speed and pitch angles are plotted as a function of time. Dashed line marks the 5 mm/s threshold for bout detection. Cyan vertical lines label time of the peak speed for each bout.

See also Table S2 and Videos S2 and S3.