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Effect of fadrozole on vitellogenic growth in bmp15 and inha double mutant. (A) Morphology and histology of the double mutant gonads (bmp15-/-;inha-/-) after fadrozole treatment. The females were reared in 10 L tanks from 80 to 100 dpf with dried powder feed containing fadrozole (200 μg/g diet) at 10% (W/W) of fish body weight per day. The resumption of follicle development to vitellogenic stage in the double mutants was completely abolished by treatment with fadrozole. The numbers shown in each sample indicate total number of fish sampled (lower) and the number of fish that showed the same phenotype (upper). (B) Expression of cyp19a1a in the PG and PV follicles of the control and mutants after E2 and fadrozole treatments. (C) Expression of vtg1 and vtg3 in the liver of the control and mutants after E2 and fadrozole treatments at 120 dpf. The relative mRNA levels were determined by real-time qPCR, normalized to the housekeeping gene ef1a, and expressed as fold change compared with the levels in the control fish. (D) Levels of E2 and Vtg in the serum of the control and mutants after E2 and fadrozole treatments. The values are expressed as mean ± SEM (n ≥ 3) and analyzed by ANOVA followed by the Tukey HSD for multiple comparisons. Different letters indicate statistical significance (P < 0.05).
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