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Expression of selected genes at PG and PV stages in bmp15 mutant. (A) Stage-matching ovarian samples collected at 60 dpf. Both PG and PV follicles were present in bmp15+/- and bmp15-/- ovaries. (B) Expression of selected genes at PG and PV stages in bmp15+/- and bmp15-/- fish. The expression of cyp19a1a and inhbaa were dramatically reduced in PV follicles of bmp15-/- fish. cyp19a1a, ovarian aromatase; fshr and lhcgr, FSH and LH receptors; inhbaa, inhbab and inhbb, activin/inhibin β subunits; inha, inhibin α subunit; fsta and fstb, follistatins. The relative mRNA levels were determined by real-time qPCR, normalized to the housekeeping gene ef1a, and expressed as fold change compared with the levels at the PG stage of the control fish. The values are expressed as mean ± SEM (n = 5) and analyzed by ANOVA followed by the Tukey HSD for multiple comparisons (*** P < 0.001; n.s., no significance). (C) Levels of E2 and Vtg in the serum of bmp15+/- and bmp15-/- females at 120 dpf. The E2 and Vtg contents of bmp15-/- were both significantly lower than those in the control. The values are expressed as mean ± SEM (n = 5) and analyzed by t-test (** P < 0.01; *** P < 0.001).
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