FIGURE

Fig. 5.

ID

ZDB-FIG-230916-213

Publication

Darroch et al., 2023 - Infection-experienced HSPCs protect against infections by generating neutrophils with enhanced mitochondrial bactericidal activity

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Fig. 5.

Infection-experienced HSPCs generate neutrophils with elevated bactericidal activity.

(A) Live confocal imaging of HSPCs in the CHT of 3 dpf Tg(Runx1:EGFP) larvae, 1 day following injection with PBS or Sal-GFP. (B) Flow quantification of HSPCs from dissected trunks of Tg(Runx1:EGFP) larvae 1 day following injection with PBS or Sal-GFP into the hindbrain ventricle (HB) or circulation (Circ.), n = 20 larvae per sample, three experimental replicates. (C) Schematic illustrating transplantation strategy to live image neutrophils derived from infection-naïve and infection-experienced HSPCs. (D) Bacterial killing rates of neutrophils derived from infection-naïve and infection-experienced HSPCs. Green data points highlight killing rates of neutrophils as shown in fig. S6H. Error bars, mean ± SD; *P < 0.05, **P < 0.01; one-way ANOVA with Tukey’s multiple comparisons test (B) and unpaired Student’s t test (D). Scale bar, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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