Fig. 1.

EG larvae have elevated survival to subsequent bacterial challenge and have neutrophils with enhanced bactericidal activity.

(A and B) Schematic illustrating strategy to generate SS (A) and EG (B) larvae. (C and D) Live imaging of the AGM/CHT regions within Tg(lyz:DsRED2) larvae at 1 and 2 dpi with PBS (C) or Sal-GFP (D). (E and F) Kaplan-Meier graphs showing survival of SS and EG larvae over 5 dpsi with Sal-GFP at 4 dpf (E) and 6 dpf (F). (G and H) Bacterial burdens within individual SS or EG larvae at 3, 6, and 24 hpsi with Sal-GFP at 4 dpf (G) and 6 dpf (H). (I) Bacterial killing rates of SS and EG neutrophils following Sal-GFP infection. Green data points highlight killing rates of neutrophils as shown in fig. S1D. (J) Schematic illustrating injection of SS and EG larvae with Sal-GFP and CellROX. (K) Quantification of ROS production within Sal-GFP-laden SS and EG neutrophils, as detected by CellROX fluorescence. Green data points highlight ROS production of neutrophils as shown in fig. S1G. Error bars, mean ± SD; ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; Gehan-Breslow-Wilcoxon test (E and F) and unpaired Student’s t test (G, H, I, and K). Scale bars, 50 μm.