FIGURE

Fig. 9

ID

ZDB-FIG-230911-9

Publication

Das et al., 2023 - Self-transfecting GMO-PMO chimera targeting Nanog enable gene silencing in vitro and suppresses tumor growth in 4T1 allografts in mouse

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Fig. 9

Uptake efficiency and mode of cellular entry

(A) Uptake efficiency of NGP ASO, NGP Scr, NP ASO, and Bodipy. (B) NGP ASO shows dose-dependent increase in cellular entry to reach 100% saturation at 50 nM. (C) Time-dependent increase in uptake efficiency; 100% saturation after 1-h exposure duration at both 500 and 1,000 nM. (D) Flow cytometry results of NGP ASO-BODIPY uptake after use of chloroquine, chlorpromazine, amiloride, and genistein. (E) Western blot analysis of NANOG in MCF7 cells after treatment with NGP ASO/NPASO alone and in combination with chloroquine. (F) Immunoblot analysis of NANOG in MCF7 cells after treatment with NGP ASO/NPASO alone and in combination with amiloride, genistein, and chlorpromazine. Data presented as mean � SEM. ?p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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