Fig. 6

Biodistribution of radiolabeled PAP3 liposomes in mice. a) Representative positron emission tomography (PET) images (coronal projections) obtained at 0–1, 1–5, 5–10, 10–30 min, and 2 and 6 h after injection of radiolabeled PAP3 liposomes containing 0.2 mol% of ⁶⁴Cu-NOTA-Bz-SCN-C18 in 6–8 weeks male mice treated (or not treated) with XEN445 (30 mg kg⁻¹ orally, b.i.d. for 9 days) prior to liposomal injection (n = 2 per group and timepoint). PET images have been co-registered with representative CT slices for anatomical localization of the radioactive signal. b) Ex vivo gamma radiation counter of plasma in mice treated (or not treated) with XEN445. Concentration of radioactivity in plasma at 10 min, 2h, and 6h after i.v. administration of radiolabeled PAP3 liposomes (n = 3 per time point). c) Ex vivo gamma radiation counter of liver and spleen in mice treated (or not treated) with XEN445 at 10 min, 2h, and 6h after i.v. administration of radiolabeled PAP3 liposomes (n = 3 per group and time point). Statistical significance was evaluated using a two-tailed unpaired Student's t-test. ns: not significant (p > 0.05). Significantly different *p < 0.05, **p < 0.01; ***p < 0.001. Exact p-values for b: 0.2379, 0.0242, and 0.1095. Exact p-values for c: 0.0259, 0.0029, and 0.0103 at 10 min, 2 h, and 6 h respectively for the liver and 0.5668, 0.5323, and 0.4659 for the spleen.