FIGURE

Fig. 5

ID
ZDB-FIG-230906-17
Publication
Arias-Alpizar et al., 2022 - Phase-separated Liposomes Hijack Endogenous Lipoprotein Transport and Metabolism Pathways to Target Subsets of Endothelial Cells in vivo
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Fig. 5

Internalization of PAP3 in zebrafish bECs. a) Timeline of injection and imaging. b,c) Biodistribution (10x and 40x magnification) of PAP3 liposomes (5 mM, 0.2 mol% DOPE-LR) after post-administration of heparin (1 nl, 50 mg ml−1) in a Tg(kdrl:GFP) zebrafish embryo at 1.5 hpi. d) Timeline of injection and imaging. e,f) Biodistribution of PAP3 liposomes (10 mM), containing 0.5 mol% of pH-sensitive DOPE-pHrodo (green/grey) to indicate endocytosis and 0.5 mol% of non-sensitive pH dye DOPE-NBD (magenta/grey) to label liposomes, in an AB/TL zebrafish embryo at 1.5 hpi. All zebrafish larvae at ≈78 hpi. Liposomes formulated by extrusion. Scale bars: 200 µm (whole embryo) and 50 µm (tissue level).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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