FIGURE

Fig. 5

ID

ZDB-FIG-230830-12

Publication

Zhong et al., 2022 - Exogenous iron impairs the anti-cancer effect of ascorbic acid both in vitro and in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Iron protected AA-induced mitochondrial dysfunction and ER stress. (A-D) A549 cells were treated with AA (2 mM, 2 h) with or without pretreatment of FeSO₄, FeCl₃, FAC, or hemin, respectively. Then (A) mROS and (B) mitochondria Ca²⁺ were detected with flow cytometry, (C) MMP was evaluated with JC-1 staining, (Bar = 100 μm), (D) mitochondria swelling was monitored with MitoTracker Green. (Bar = 10 μm). (E) A549 cells were treated with AA (2 mM, 2 h) with or without pretreatment of FeSO₄, FeCl₃, FAC, or hemin, respectively, and P-eIF2α, eIF2α were detected. β-actin served as the loading control. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Adv Res