FIGURE

Fig. 4

ID

ZDB-FIG-230830-11

Publication

Zhong et al., 2022 - Exogenous iron impairs the anti-cancer effect of ascorbic acid both in vitro and in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Iron protected AA-induced cell death. (A-B) A549 cells were treated with AA (2 mM, 4 h) with or without pretreatment of FeSO₄, FeCl₃, FAC, or hemin, respectively, then washed with PBS. After 20 h, the MTT assay was performed, and the morphological changes were acquired with a microscope. (C) A549 cells were treated with AA (2 mM, 4 h) with or without pretreatment of CuCl, CuCl₂, MgSO₄, ZnCl₂, or AlCl₃, respectively, then washed with PBS. The MTT assay was performed after 20 h. (D-G) A549 cells were treated with AA (2 mM, 2 h) with or without pretreatment of FeSO₄, FeCl₃, FAC, or hemin, respectively, and (D) ROS generation, (E) Ca²⁺ level, or (F) lipid peroxidation was detected with flow cytometry. (G) Lipid peroxidation was also detected with a fluorescence microscope. Bar = 100 μm. ^**p < 0.01. ^***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Adv Res