FIGURE

Figure 2

ID

ZDB-FIG-230828-50

Publication

Lessey et al., 2023 - Pharmacological and Genetic Disruption of C-Type Natriuretic Peptide (nppcl) Expression in Zebrafish (Danio rerio) Causes Stunted Growth during Development

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Figure 2

(A–F) Wholemount in situ hybridization of nppcl in 96 hpf wild-type zebrafish embryos. Zebrafish embryos were captured at 96 hpf and fixed with 4% PFA before wholemount in situ hybridization was performed to assess spatial expression of nppcl alongside sense probe controls. Images were captured at 20× (A,B) and 5x magnification (C–F) using bright-field microscopy on an Axiovert 135 inverted microscope (Carl Zeiss Ltd., Cambridge, UK) and DC500 color camera. In situ hybridization was performed using 12–15 embryos for each RNA probe and images are representative of the staining observed in multiple embryos. (PPH—presumptive pituitary and hypothalamus; F—forebrain; M—midbrain; H—hindbrain).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.