FIGURE
Fig. 3
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- ZDB-FIG-230818-77
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- Tang et al., 2023 - Serotonin/5-HT7 receptor provides an adaptive signal to enhance pigmentation response to environmental stressors through cAMP-PKA-MAPK, Rab27a/RhoA, and PI3K/AKT signaling pathways
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Fig. 3
Effects of 5-HT7R agonist (LP-12) and antagonist (SB269970) on melanin synthesis in two zebrafish models. (A) Schematic drawing of the experimental procedure on LP-12-induced pigmentation in zebrafish vivo model. (B) Left, zebrafishes were treated with PTU-2 (PTU removal and water given) at 35 or other five concentrations of LP-12 (0.1, 1, 10, 50, and 100 ?M) and then observed under a stereomicroscope (MI16) in lateral and dorsal views of the embryos at 60 h. Right, the relative melanin content was calculated by Image J (n = 30). ***p < .001, compared with the PTU-2. (C) Observation of LP-12 or LP-12 combining with SB269970 induced melanogenesis in the zebrafish, compared with PTU-2 (PTU always treating). (D) Schematic drawing of the experimental procedure on 5-HT-induced pigmentation in 5-HT7R gene knockdown zebrafish model. (E) Left, zebrafishes were respectively injected Cas9 protein or htr7a-sgRNA or htr7b-sgRNA or htr7a-sgRNA + htr7b-sgRNA protein, then treated with PTU (PTU removal and water given) at 35 h and 5-HT administration, then observed under a stereomicroscope (MI16) in lateral and dorsal views of the embryos at 60 h. Right, the relative melanin content was calculated by Image J. ***p < .001, compared with the 5-HT + Si-Ctrl. Each value represents the mean ± SEM (n = 5).
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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