FIGURE

Figure 3-supplement 1.

ID
ZDB-FIG-230814-228
Publication
Barlow et al., 2023 - The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
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Figure 3-supplement 1.

CRISPR-generated dmist mutants have reduced dmist transcript levels.

(A) RT-qPCR shows dmisti8/i8 larvae have reduced dmist mRNA levels, suggesting that dmisti8 transcripts undergo nonsense mediated decay. Data are mean ± SEM of three biological replicates. **p<0.01; one-way ANOVA, Tukey’s post hoc test. (B) Relative expression level of dmist transcript from RNA sequencing of 6 dpf dmisti8/i8 and dmist+/+ siblings. Z-score calculated by subtracting mean expression and normalising by the standard deviation across all expressed transcripts. Data are mean ± SEM for three independent biological replicates. **p<0.01, Student’s t-test.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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