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Figure 3-supplement 1.

ID: ZDB-IMAGE-230814-233
Publication: Barlow et al., 2023 - The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
All Figures: Figures for Barlow et al., 2023

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Figure 3-supplement 1. CRISPR-generated dmist mutants have reduced dmist transcript levels.

(A) RT-qPCR shows dmist^i8/i8 larvae have reduced dmist mRNA levels, suggesting that dmistⁱ⁸ transcripts undergo nonsense mediated decay. Data are mean ± SEM of three biological replicates. **p<0.01; one-way ANOVA, Tukey’s post hoc test. (B) Relative expression level of dmist transcript from RNA sequencing of 6 dpf dmist^i8/i8 and dmist^+/+ siblings. Z-score calculated by subtracting mean expression and normalising by the standard deviation across all expressed transcripts. Data are mean ± SEM for three independent biological replicates. **p<0.01, Student’s t-test.

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