Figure 3-supplement 1.

Figure 3-supplement 1. CRISPR-generated dmist mutants have reduced dmist transcript levels.

(A) RT-qPCR shows dmist^i8/i8 larvae have reduced dmist mRNA levels, suggesting that dmistⁱ⁸ transcripts undergo nonsense mediated decay. Data are mean ± SEM of three biological replicates. **p<0.01; one-way ANOVA, Tukey’s post hoc test. (B) Relative expression level of dmist transcript from RNA sequencing of 6 dpf dmist^i8/i8 and dmist^+/+ siblings. Z-score calculated by subtracting mean expression and normalising by the standard deviation across all expressed transcripts. Data are mean ± SEM for three independent biological replicates. **p<0.01, Student’s t-test.