PUBLICATION
The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
- Authors
- Barlow, I.L., Mackay, E., Wheater, E., Goel, A., Lim, S., Zimmerman, S., Woods, I., Prober, D.A., Rihel, J.
- ID
- ZDB-PUB-230810-41
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Barlow, Ida, Prober, David, Rihel, Jason, Woods, Ian G., Zimmerman, Steve
- Keywords
- genetic screen, neuroscience, sleep, sleep homeostasis, sodium-potassium pump, zebrafish
- MeSH Terms
-
- Phosphoproteins/metabolism
- Sleep/genetics
- Sodium-Potassium-Exchanging ATPase/genetics
- Sodium-Potassium-Exchanging ATPase/metabolism
- Homeostasis
- PubMed
- 37548652 Full text @ Elife
Abstract
Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na+,K+-ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a, a Na+,K+-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na+ levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na+ pump function modulates neuronal excitability to maintain normal sleep behaviour.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping