Figure 5

Mcm10 overexpression increases the proliferation of emerging HSCs at the level of the hemogenic endothelium

(A) Fluorescence imaging of dorsal aorta in 32-hpf kdrl:mcherry/cmyb:GFP double-transgenic embryos, either non-injected or injected with mcm10 full-length mRNA.

(B) The number of double-positive cells was reported for each condition. Statistical analysis was completed using an unpaired two-tailed t test. ^∗∗∗p < 0.0001. Center values denote the mean, and error values denote SEM (n = number of total embryos from three independent experiments).

(C) An immunofluorescence against GFP and phospho-histone 3 (pH3) was performed on 32-hpf flk1:GFP transgenic embryos, either injected with control or mcm10 morpholinos, and with mcm10 full-length mRNA. The aorta region was imaged and (D) the number of double-positive cells (representing the proliferating endothelial cells) was scored for all embryos in each condition. Center values denote the mean, error values denote SEM, and statistical analysis was completed using ANOVA multiple t test. ^∗p < 0.01, ^∗∗p < 0.001 (n = number of total embryos from three independent experiments). Scale bar: 50 μm (A–C).