Fig. 3

GABAergic neurons co-express tal2, gata3, gata2a, and nkx1.2lb factors in the diencephalon and nMLF. (A-A?) Heads of wild-type of 30 hpf zebrafish hybridized to gad67 (A) and tal2 (A?) antisense probes, a merged view (A?), and a transverse section at the level of cell marked with asterisk (A?). gad67 expressing cells are tal2-positive in the diencephalon and nMLF with a varying level. (B-B?) GABA immunohistochemistry (B), tal2 in situ hybridization (B?), a merged view (B?), and a transverse section at the level of cell marked with asterisk (B?). Almost all GABAergic neurons co-express tal2 mRNA in the nMLF (plus). (C-C?) gad67 (C), gata3 (C?) expressing cells, merge (C?), and a transverse section at the level of cell marked with asterisk (C?). gad67 mRNA expressing cells co-express gata3 at the single cell level (C1 to C1?). (D-D?) gata3 in situ hybridization (D), GABA immunohistochemistry (D?), a merged view (D?), and a transverse section at the level of cell marked with asterisk (D?). All GABAergic neurons co-express gata3 mRNA in the nMLF (asterisk). (E-E?) gata2a in situ hybridization (E), GABA immunohistochemistry (E?), a merged view (E?), and a transverse section at the level of cell marked with asterisk (E?). Many GABAergic neurons co-express gata2a mRNA in the nMLF (stars). (F-F?) gad67 (F), nkx1.2lb (F?) expressing cells, merge (F?), and views at the level of single cell (F1 to F1?). gad67 mRNA expressing cells co-express nkx1.2lb. (G-G?) nkx1.2lb (G), GABA immunohistochemistry (G?), a merged view (G?), and views at the level of single cell (G1 to G1?). All GABAergic neurons co-express nkx1.2lb mRNA (asterisk). (A-F?) projections of single section. Dashed regions in main panels (C-C?) and (F-G?) are shown in magnified view in panels (C1-G1?). All embryos' eyes were removed by dissection. Dorsal is up; rostral, to the left. Scale bar: 25 ?m