PUBLICATION
Tal2 is required for generation of GABAergic neurons in the zebrafish midbrain
- Authors
- Wang, R., Guo, S., Yang, L.
- ID
- ZDB-PUB-220907-14
- Date
- 2022
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 252(2): 263-275 (Journal)
- Registered Authors
- Keywords
- GABAergic interneuron, Transcription factor, Zebrafish midbrain, gata2a, gata3, tal2
- MeSH Terms
-
- Animals
- Cell Differentiation
- GABAergic Neurons
- Mesencephalon*/metabolism
- Mice
- Neurogenesis/genetics
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 36063149 Full text @ Dev. Dyn.
Citation
Wang, R., Guo, S., Yang, L. (2022) Tal2 is required for generation of GABAergic neurons in the zebrafish midbrain. Developmental Dynamics : an official publication of the American Association of Anatomists. 252(2):263-275.
Abstract
Background In the zebrafish midbrain, GABAergic neurons develop from precursors located in the nucleus of the medial longitudinal fasciculus (nMLF). However, the precise mechanisms that underline generation of the nMLF GABAergic neuron are poorly understood.
Results GABAergic neurons in the nMLF co-express transcription factors tal2, gata2a, gata3, and nkx1.2lb. The Nodal-related gene and shh signaling are required for differentiation of nMLF GABAergic neuron precursors. Tal2 is important for nMLF GABAergic neurogenesis. Disruption of Tal2, embryos completely lack the GABA-synthesizing enzyme glutamic acid decarboxylase 67 gene (gad67) expressing cells in the nMLF, and the whole nkx1.2lb expressing cells in the midbrain. Although almost all tal2-expressing cells in the diencephalon and/or nMLF are gata2a- and gata3-positive, simultaneous knockdown of gata2a and gata3 does not affect either tal2 or gad67 expression.
Conclusions In the zebrafish midbrain, expression of tal2, gata2a and/or gata3 is independent of each other. The function of gata2a and gata3 is dispensable for generation of GABAergic neuron in the nMLF. This suggests that the functional connections of the regulatory genes leading to generation of nMLF GABAergic neurons have diverged between mouse and zebrafish. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping