Figure 7

The nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) inhibitor (nfkbiab) transcript was upregulated in whole bodies of anxiety-induced middle-aged adult males and females. Anxiety-like behavioral tests: (A) crossings to upper half of tank, (B) percent of the time in the upper half of tank (C) height above the bottom of the tank. NO F, CAF F—non-treated and caffeine-exposed females; NO M, CAF M—non-treated and caffeine-exposed males. n = 5 male and n= 7–9 female fish in three independent experiments (each experiment had both males and females). Student’s t-test, *—p < 0.05, **—p < 0.01. (D) Scheme showing that individual fish were exposed to water or water containing caffeine in the tank for 15 min, during which movements were recorded. NO—no treatment; CAF—caffeine exposure. (E) qRT-PCR results: n= 4–6 male fish and 5–7 females in each treatment; two independent experiments, with males and females in each. The reference genes were actb2 and eef1a1l1. Student’s t-test.