CD271 activation increases response to chemotherapy and promotes macrophage recruitment in zebrafish a CD271 expression in SCC13 cells treated with Cisplatin (5μg/mL) vs control by FACS. b Representative images of SCC13 cells (red) injected into zebrafish at 7dpi after treatments as described. Scale bar = 500µm. c cSCC metastasis quantified and classified as Full metastases, Initial metastases, or In place. d-e Quantification of the red fluorescence intensity of tumor mass in zebrafish by ImageJ software and ImageJ mask. f Left images: schematic representation of mpeg1 positive cells (red) in zebrafish injected with mock or CD271-overexpressing cells (green) within the analyzed area (blue). Right images: schematic representation of treatments. g Quantification of mpeg1-positive cells in zebrafish injected with mock or CD271-overexpressing cells and h in zebrafish injected with SCC13 cells after treatments as described, by whole-mount in situ hybridization. PBS was used as a control (n = 3, 3 independent evaluations). Results are shown as the mean percentage of three independent experiments (minimum of 20 injected zebrafish/condition). Statistical analysis was performed using the two-way ANOVA and multiparametric T-test. *0.01 < p < 0.05, **0.001 < p < 0.01, ***0.0001 < p < 0.001, ****p < 0.0001
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