FIGURE

Figure 4

ID

ZDB-FIG-230711-24

Publication

Lescouzères et al., 2023 - A multilevel screening pipeline in zebrafish identifies therapeutic drugs for GAN

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Figure 4

Rescreen identifies 59 compounds restoring locomotion defects in the gan^del/del model

The 103 Hits identified in the gan MO‐injected fish were further tested in the gan CRISPR zebrafish line at 5 dpf. Due to the great imbalance toward positive results (all 103 compounds are Hits), the statistical analysis used a normalization of Percent Activation (%). Readout signals were calibrated and normalized to a 0–100% effect scale, in which 0% represents no effect and 100% corresponds to the locomotion activity of WT fish (See details in Appendix Table S1). Here, the cutoff for the restoration of motility was assigned to > 50% and identifies 59 Hits. Data information: For each drug, circles represent individual larvae (n = 4) and red lines show the medians for the Normalized Percent Activation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.