elna mutants have a shorter life expectancy. (A) Zebrafish survival from 3 independent clutches resulting from the crossing of heterozygous (elna sa12235/+) fish was recorded after genotyping, from 10–11 mpf to 15–22 mpf (WT = 27; HET = 78; MUT = 41 zebrafish). Survival curves were compared using the log-rank Mantel-Cox test and the Log-rank test for trend; * p < 0.05. (B,C) Weight and size of fish were measured at 11 and 20 mpf and expressed as a body mass index (BMI). Data were analyzed by Kruskal–Wallis test; ns = non-significant; mpf = months post fertilization; WT = wild type (elna+/+); HET = heterozygous (elna sa12235/+); MUT = mutant (elna sa12235/ sa12235).
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