FIGURE

Fig. 6

ID

ZDB-FIG-230622-44

Publication

Zhang et al., 2022 - ST3GAL5-catalyzed gangliosides inhibit TGF-β-induced epithelial-mesenchymal transition via TβRI degradation

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Fig. 6

ST3GAL5 promotes TβRI ubiquitination and degradation

HEK293T cells transfected with Myc‐tagged caTβRI, (HA‐Ub, and nontargeting siRNA or ST3GAL5 siRNA were collected for IP with an anti‐Myc antibody and immunoblot analysis. All groups were treated with MG132 (5 μM) for 6 h.
Ubiquitination of TβRI was detected by IP of Myc‐tagged caTβRI from HA‐Ub‐transfected HEK293T cells with or without ST3GAL5 overexpression. All groups were treated with MG132 (5 μM) for 6 h.
Western blot analysis of TβRI expression in A549‐VIM‐RFP cells transduced with the pLV‐EV control or ST3GAL5 expression construct and treated with CHX for the indicated times. Vinculin: loading control.
Quantification of TβRI expression in pLV‐EV control‐ and ST3GAL5‐expressing A549‐VIM‐RFP cells. The results were normalized to the t = 0 controls. The data are shown as the mean ± SD of three independent experiments. **P < 0.01 based on two‐way ANOVA.
Working model for UGCG–ST3GAL5–GM3‐mediated inhibition of TGF‐β signaling via induction of TβRI localization in lipid rafts. The dashed arrow indicates that the SMAD activation‐induced decrease in ST3GAL5 expression has not been formally shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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