FIGURE

Fig. 3

ID

ZDB-FIG-230614-3

Publication

Grissenberger et al., 2022 - High-content drug screening in zebrafish xenografts reveals high efficacy of dual MCL-1/BCL-X_L inhibition against Ewing sarcoma

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Fig. 3

Fig. 3. Single agent treatment of xenotransplanted larvae A) Schematic representation of the workflow - Zebrafish embryos were xenotransplanted with shSK-E17T Ewing sarcoma cells at 2 dpf. After 24 h (1 dpi) embryos were imaged on the Operetta CLS and subsequently incubated in respective amounts of small compounds. After 48 h (3 dpi) larvae were imaged again and changes in tumor size were analyzed. B-E) Xenotransplanted larvae were treated with irinotecan (100 ?M, B), S63845 (10 ?M, C), A-1331852 (10 ?M, D) and YK-4-279 (10 ?M, E) for 48 h (1 dpi - 3 dpi). Dot plots show relative changes of tumor area (3 dpi/1 dpi). Waterfall plots (right panels, 1 bar per zebrafish) total change in tumor area (3 dpi - 1 dpi). Scale bar is 125 ?m. Statistical analyses were performed with a Mann-Whitney test, ****: p ? 0.0001, ***: p ? 0.001. Error bars represent SEM of combined larvae from two independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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