FIGURE

Fig. 5

ID

ZDB-FIG-230605-21

Publication

Lumaquin-Yin et al., 2023 - Lipid droplets are a metabolic vulnerability in melanoma

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Knockout of DGAT1 suppresses lipid droplet formation and tumor progression.

a Representative fluorescent images of ZMEL-LD lipid droplets marked by PLIN2-tdTOMATO. Cells were incubated with 100 µM oleic acid for 24 h and lipid droplets were quantified via flow cytometry (mean ± SEM, n = 4 biologically independent experiments). Statistics via two-tailed t-test with Holm-Sidak correction. b Schematic of zebrafish TEAZ. Transgenic casper;mitfa:BRAF^V600E;p53^−/− zebrafish were injected with tumor-initiating plasmids and electroporated to generate BRAF^V600E p53^−/− PTEN^ko melanomas with normal or suppressed lipid droplet formation. c Representative images of zebrafish flank with TEAZ-generated tumors. Corresponding quantification of tumor area via image analysis as described in Methods (mean ± SEM, n = 3 biologically independent injections, sgNT n = 45, sgDGAT1a n = 49). Statistics via two-sided Mann-Whitney U test at each time point. d Representative histological images from week 12 TEAZ generated tumors with H&E, BRAF^V600E, and PLIN2 staining (n = 4 fish per genotype). Yellow arrows denote punctate PLIN2 staining for lipid droplets. Figure 5b was created with Biorender.com.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.