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Fatty acids are utilized by melanocytic cells for oxidative metabolism. Fatty acid oxidation gene score for undifferentiated/neural crest (U/NC), transitory (T), and melanocytic (M) melanomas in the databases from: a CCLE (Tukey’s boxplot center = median, box bounds = 25th and 75th percentile, whiskers = min and max, n = number of independent cell lines) and b TCGA (Violin plot with bar indicating mean, n = number of patient samples). Statistics via two-sided Wilcoxon rank sum test with Holm correction. *** p < 0.001, **** p < 0.0001. Fatty acid oxidation stress test in A375 cells showing fold change normalized OCR: c basal OCR from measurement 3 and d etomoxir response from measurement 9 (Tukey’s boxplot center = median, box bounds = 25th and 75th percentile, whiskers = min and max, n = 3 biologically independent experiments). Statistics via two-tailed t-test with Holm-Sidak correction. * p = 0.013, *** p < 0.001, **** p < 0.0001. e Fold change in fatty acid uptake in drug-treated human A375 cells (mean ± SEM, n = 3 biologically independent experiments). Statistics via area under the curve two-tailed t-test with Holm–Sidak correction. * p < 0.05. f Heatmap of CCLE melanocytic gene expression from RPMI-7951 (U/NC), A2058 (T), SKMEL5 (M), and SKMEL28 (M) cells. g Fatty acid uptake (FAU) velocity from RPMI-7951 (U/NC), A2058 (T), SKMEL5 (M), and SKMEL28 (M) cells (mean ± SEM, n = 13 biologically independent replicates over n = 3 biologically independent experiments). Statistics via two-tailed t-test. * p = 0.03, *** p = 0.0006.
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