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Figure 6

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ZDB-FIG-230531-25

Publication

Zhao et al., 2023 - The manipulation of cell suspensions from zebrafish intestinal mucosa contributes to understanding enteritis

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Figure 6

Flow cytometric analysis of transgenic zebrafish, including Tg(lyz:DsRED2), Tg(rag2:DsRed), Tg(lck:EGFP), and Tg(mpeg1:EGFP). (A) The gating strategies in cytometric analysis for intestinal neutrophils (with red fluorescence labeled Lyz), immature lymphocytes (with red fluorescence labeled Rag2), mature T lymphocytes (with green fluorescence labeled Lck), and macrophages (with green fluorescence labeled Mpeg1) in zebrafish. Compare with wild type samples, all types of fluorescence labeled immune cells could be accurately sorted. (B) Comparing samples made by mechanical dissociation of whole intestine and blowing off mucosal cells from muscularis, the fluorescence labeled cells were significantly enriched. * represented p < 0.05, **** represented p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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